CD30 (H559) polyclonal antibody

Product Specifications

Background

The tumor necrosis factor (TNF) receptor family is composed of several type I integral membrane glycoproteins that exhibit homology in their cystine-rich extracellular domains. Members of this family include FAS, OX40, CD27 and CD30. CD30 is a surface marker for neoplastic cells of the Hodgkin's lymphoma and related hematologic malignancies.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Tumor necrosis factor receptor superfamily member 8; CD30L receptor; Ki-1 antigen; Lymphocyte activation antigen CD30; NFRSF8; CD30; D1S166E

Swiss Prot

P28908

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 500-600 of Human CD30.

Conjugation

Unconjugated

Applications

WB IHC IP

Dilution

WB 1:500 - 1:1000 IHC 1:50 - 1:100 IP 1:10 - 1:100

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 96% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 120 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

CD30 (H559) polyclonal antibody detects endogenous levels of CD30 protein.

Applications Notes

Western blot (WB) analysis of CD30 (H559) polyclonal antibody at 1:1000 dilution Lane1:HCT116 whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (40ug) Lane3:CT26 whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug) Lane5:The Testis tissue lysate of Rat (40ug)