CD19 (D424) polyclonal antibody
Product Specifications
Background
CD19 is a transmembrane glycoprotein that contains two extracellular immunoglobulin-like domains. CD19 is selectively expressed on the cell surface of B-lymphocytes, where it activates intracellular signaling cascades involving both Ras and phosphatidylinositol 3-kinase pathways. Activation of CD19 results in cross-linking of the membrane protein immunoglobulin chains and the subsequent association with Src family protein tyrosine kinases (PTK) . Expression of CD19 is continuous throughout B-cell development and through terminal differentiation of B-cells into plasma cells. CD19 forms functional complexes with B-lymphocyte surface proteins, including integrin β1, CD21 and CD81, which are involved in regulating B-cell development.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Differentiation antigen CD19; T-cell surface antigen Leu-12
Swiss Prot
P15391
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 400-450 of Human CD19.
Conjugation
Unconjugated
Related Pathways
Tumor-Infiltrating Immune Cell Marker Guide, B cell receptor signaling
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 96% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 80 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
CD19 (D424) polyclonal antibody detects endogenous levels of CD19 protein.
Applications Notes
Western blot (WB) analysis of CD19 (D424) polyclonal antibody at 1:500 dilution Lane1:THP-1 whole cell lysate (20ug) Lane2:Jurkat whole cell lysate (20ug) Lane3:HEK293T whole cell lysate (20ug) Lane4:The spleen tissue lysate of Mouse (40ug) Lane5:The spleen tissue lysate of Rat (40ug)
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
orb2930195-01 20 µg
Recombinant Drosophila melanogaster Transmembrane protein 11 homolog, mitochondrial (Pmi)
Sign In for Pricing
View Details orb2930195-02 100 µg
Recombinant Drosophila melanogaster Transmembrane protein 11 homolog, mitochondrial (Pmi)
Sign In for Pricing
View Details 