CD19 (D424) polyclonal antibody

CD19 is a transmembrane glycoprotein that contains two extracellular immunoglobulin-like domains. CD19 is selectively expressed on the cell surface of B-lymphocytes, where it activates intracellular signaling cascades involving both Ras and phosphatidylinositol 3-kinase pathways. Activation of CD19 results in cross-linking of the membrane protein immunoglobulin chains and the subsequent association with Src family protein tyrosine kinases (PTK) . Expression of CD19 is continuous throughout B-cell development and through terminal differentiation of B-cells into plasma cells. CD19 forms functional complexes with B-lymphocyte surface proteins, including integrin β1, CD21 and CD81, which are involved in regulating B-cell development.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 96% (by SDS-PAGE) .

Western blot (WB) analysis of CD19 (D424) polyclonal antibody at 1:500 dilution Lane1:THP-1 whole cell lysate (20ug) Lane2:Jurkat whole cell lysate (20ug) Lane3:HEK293T whole cell lysate (20ug) Lane4:The spleen tissue lysate of Mouse (40ug) Lane5:The spleen tissue lysate of Rat (40ug)