ATG10 (P220) polyclonal antibody

Product Specifications

Background

Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608) -ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Ubiquitin-like-conjugating enzyme ATG10; Autophagy-related protein 10; APG10-like; APG10L; PP12616

Swiss Prot

Q9H0Y0

Reactivity

Human

Immunogen

Synthetic peptide, corresponding to amino acids 200-245 of Human ATG1.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 96% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 30 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

ATG10 (P220) polyclonal antibody detects endogenous levels of ATG10 protein.

Applications Notes

Western blot (WB) analysis of ATG10 (P220) polyclonal antibody at 1:500 dilution Lane1:Hct116 whole cell lysate (40ug) Lane2:786-O whole cell lysate (40ug) Lane3:SGC-7901 whole cell lysate (40ug) Lane4:HEK293T whole cell lysate (40ug)