VEGF-A (L98) polyclonal antibody
Product Specifications
Background
VEGF is a dimeric glycoprotein with structural homology to PDGF. Several variants of VEGF have been described that arise by alternative mRNA splicing. It has been speculated that VEGF may function as a tumor angiogenesis factor in vivo because the expression pattern of VEGF is consistent with a role in embryonic angiogenesis. VEGF mRNA is formed in some primary tumors, VEGF is produced by tumor cell lines in vitro and VEGF mitogenic activity appears to be restricted to endothelial cells. A member of the PDGF receptor family, Flt, has been identified as a high-affinity receptor for VEGF.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Vascular endothelial growth factor A; VEGF-A; Vascular permeability factor; VPF; VEGFA; VEGF
Swiss Prot
P15692
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 70-120 of Human VEGF206.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 27 kDa (VEGF206) ; 45 kDa (L-VEGF206) ; ~ 15-44 kDa (Others Isoform)
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
VEGF-A (L98) polyclonal antibody detects endogenous levels of total VEGF-A protein (13 isoforms) .
Applications Notes
Western blot (WB) analysis of VEGF-A (L98) pAb at 1:500 dilution Lane1:LOVO whole cell lysate (40ug) Lane2:Panc1 whole cell lysate (40ug) Lane3:CT26 whole cell lysate (40ug) Lane4:H9C2 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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