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VEGF-A (L98) polyclonal antibody

Product Specifications

Background

VEGF is a dimeric glycoprotein with structural homology to PDGF. Several variants of VEGF have been described that arise by alternative mRNA splicing. It has been speculated that VEGF may function as a tumor angiogenesis factor in vivo because the expression pattern of VEGF is consistent with a role in embryonic angiogenesis. VEGF mRNA is formed in some primary tumors, VEGF is produced by tumor cell lines in vitro and VEGF mitogenic activity appears to be restricted to endothelial cells. A member of the PDGF receptor family, Flt, has been identified as a high-affinity receptor for VEGF.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Vascular endothelial growth factor A; VEGF-A; Vascular permeability factor; VPF; VEGFA; VEGF

Swiss Prot

P15692

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 70-120 of Human VEGF206.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 27 kDa (VEGF206) ; 45 kDa (L-VEGF206) ; ~ 15-44 kDa (Others Isoform)

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

VEGF-A (L98) polyclonal antibody detects endogenous levels of total VEGF-A protein (13 isoforms) .

Applications Notes

Western blot (WB) analysis of VEGF-A (L98) pAb at 1:500 dilution Lane1:LOVO whole cell lysate (40ug) Lane2:Panc1 whole cell lysate (40ug) Lane3:CT26 whole cell lysate (40ug) Lane4:H9C2 whole cell lysate (40ug)

Host or Source

Rabbit

Available Sizes

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