MYLPF (phospho-S16) polyclonal antibody
Product Specifications
Background
MYLPF (myosin light chain, phosphorylatable, fast skeletal muscle), also known as fast skeletal myosin light chain 2 or MLC2B, is a 169 amino acid protein that is expressed in fetal and adult skeletal muscle. A calicum binding protein, MYLPF contains three EF hand domains and is encoded by a gene that maps to human chromosome 16p11.2. Chromosome 16 encodes over 900 genes in approximately 90 million base pairs, makes up nearly 3% of human cellular DNA and is associated with a variety of genetic disorders. The GAN gene is located on chromosome 16 and, with mutation, may lead to giant axonal neuropathy, a nervous system disorder characterized by increasing malfunction with growth.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Myosin regulatory light chain 2, skeletal muscle isoform; Fast skeletal myosin light chain 2; MLC2B; MYLPF
Swiss Prot
Q96A32
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human MYLPF around the phosphorylation site of Serine 16.
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 19 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-MYLPF (S16) polyclonal antibody detects endogenous levels of MYLPF only when phosphorylated at Ser16.
Applications Notes
Western blot (WB) analysis of p-MYLPF (S16) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate (40ug) Lane2:K562 whole cell lysate (40ug) Lane3:The Muscle tissue lysate of Rat (40ug)
Host or Source
Rabbit
Available Sizes
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