Separase (A795) polyclonal antibody

Product Specifications

Background

Separase is a cysteine protease that triggers anaphase in all eukaryotes by participating in separation of sister chromatids during mitosis. Once activated, separase hydrolyzes the SCC1 subunit of cohesin, the chromosomal protein complex responsible for sister chromatid cohesion. Separase and cohesin are highly conserved from yeasts to humans. When the cell is not dividing, separase is prevented from cleaving cohesin through its association with another protein, securin. When anaphase is signaled, the securin is ubiquitinated and hydrolyzed by APC/cyclosome, releasing the active separase. Separase is transiently activated between the two meioses and may also be involved in homolog separation.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Separin; Caspase-like protein ESPL1; Extra spindle poles-like 1 protein; Separase; ESPL1; ESP1; KIAA0165

Swiss Prot

Q14674

Reactivity

Human

Immunogen

Synthetic peptide, corresponding to amino acids 763-824 of Human Separase.

Conjugation

Unconjugated

Applications

Dilution

WB:1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 230 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Separase (A795) polyclonal antibody detects endogenous levels of Separase protein.

Applications Notes

Western blot (WB) analysis of Separase (A795) pAb at 1:500 dilution Lane1:EC9706 whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:SGC7901 whole cell lysate (40ug)