Separase (A795) polyclonal antibody
Product Specifications
Background
Separase is a cysteine protease that triggers anaphase in all eukaryotes by participating in separation of sister chromatids during mitosis. Once activated, separase hydrolyzes the SCC1 subunit of cohesin, the chromosomal protein complex responsible for sister chromatid cohesion. Separase and cohesin are highly conserved from yeasts to humans. When the cell is not dividing, separase is prevented from cleaving cohesin through its association with another protein, securin. When anaphase is signaled, the securin is ubiquitinated and hydrolyzed by APC/cyclosome, releasing the active separase. Separase is transiently activated between the two meioses and may also be involved in homolog separation.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Separin; Caspase-like protein ESPL1; Extra spindle poles-like 1 protein; Separase; ESPL1; ESP1; KIAA0165
Swiss Prot
Q14674
Reactivity
Human
Immunogen
Synthetic peptide, corresponding to amino acids 763-824 of Human Separase.
Conjugation
Unconjugated
Applications
WB
Dilution
WB:1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 230 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Separase (A795) polyclonal antibody detects endogenous levels of Separase protein.
Applications Notes
Western blot (WB) analysis of Separase (A795) pAb at 1:500 dilution Lane1:EC9706 whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:SGC7901 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
Curated Selection
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