AhR (phospho-S36) polyclonal antibody

Product Specifications

Background

The Aryl Hydrocarbon Receptor (AHR), also known as the dioxin receptor, is a ligand-activated helix/loop/helix transcription factor found in a variety of vertebrate species. The known ligands for AHR are foreign planar aromatic compounds, such as polycyclic aromatic compounds and halogenated aromatic compounds such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) .Unlike the steroid/thyroid hormone receptors, there is no known physiological ligand for the AH Receptor. Studies indicate that in non-ligand activated cells, AHR is found complexed with HSP90 predominantly in the cytoplasm. Upon binding to an agonist, the ligand-activated AHR is believed to transform to a nuclear, DNA binding form. This transformation process appears to involve dissociation of HSP90 from AHR followed by formation of a heterodimer with AHR nuclear translocator protein (Arnt) . The AHR-ligand complex appears to initiate gene transcription of cytochrome P450 1A1.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Aryl hydrocarbon receptor; Ah receptor; AhR; Class E basic helix-loop-helix protein 76; bHLHe76; AHR; BHLHE76

Swiss Prot

P35869

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human AhR around the phosphorylation site of Serine 36.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 94 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-AhR (S36) polyclonal antibody detects endogenous levels of AhR only when phosphorylated at Ser36.

Applications Notes

Western blot (WB) analysis of p-AhR (S36) at 1:2000 dilution Lane1:MCF-7 whole cell lysate (40ug) Lane2:The Heart tissue lysate of Mouse (40ug) Lane3:The Heart tissue lysate of Rat (40ug) Lane4:HepG2 whole cell lysate (20ug)