PKC θ (phospho-T538) polyclonal antibody

Product Specifications

Background

Members of the protein kinase C (PKC) family of serine/threonine kinases play a key role in regulating the differentiation and growth of diverse cell types. PKC theta is a member of the PKC family, and its predominant expression in hematopoietic cells suggests that it may play a role in signal transduction and growth regulatory pathways unique to these cells. PKC theta is a Ca (2+) -independent PKC isoform that is selectively expressed in T lymphocytes (and muscle), and it is thought to play an important role in T cell receptor-induced activation. Among several PKC isoenzymes expressed in T cells, PKC theta is unique in being rapidly recruited to the site of TCR clustering. It has been shown that PKC theta is essential for TCR-mediated T-cell activation, but it is dispensable during TCR-dependent thymocyte development.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Protein kinase C theta type; nPKC-theta; PRKCQ; PRKCT

Swiss Prot

Q04759

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human PKC θ around the phosphorylation site of Threonine 538.

Conjugation

Unconjugated

Related Pathways

Protein Kinase C Signaling, Inhibition of Apoptosis, Mitochondrial Control of Apoptosis

Applications

Dilution

WB:1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 80 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

PKC θ (phospho-T538) polyclonal antibody detects endogenous levels of PKC θ protein only when phosphorylated at Thr538.

Applications Notes

Western blot (WB) analysis of PKC θ (phospho-T538) polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate Lane2:MCF-7 treated with LPS (100ng/ml) for 5 minutes whole cell lysate Lane3:MCF-7 treated with LPS (100ng/ml) for 30 minutes whole cell lysate