Fyn (phospho-Y530) polyclonal antibody
Product Specifications
Background
Fyn is a membrane-associated, non-receptor protein tyrosine kinase approximately 59kDa, which belongs to the Src family of cytoplasmic tyrosine kinases. Fyn is very strongly similar to mouse Fyn, v-yes and c-src. Fyn is expressed predominately in tissues of neuronal and hematopoietic origin. Neuronal Fyn and hematopoietic Fyn differ at the junction of the SH2 and kinase domains due to tissue specific alternative splicing. Fyn has been shown to be involved in B cell and T cell activation as well as keratinocyte differentiation. In T cells, Fyn associates with the T-cell antigen receptor and Thy1.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Tyrosine-protein kinase Fyn; Proto-oncogene Syn; Proto-oncogene c-Fyn; Src-like kinase; SLK; p59-Fyn; FYN
Swiss Prot
P06241
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human Fyn around the phosphorylation site of Tyrosine 530.
Conjugation
Unconjugated
Related Pathways
Contribution of Soluble Factors to EMT
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 59 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-Fyn (Y530) polyclonal antibody detects endogenous levels of Fyn protein only when phosphorylated at Tyr530.
Applications Notes
Western blot (WB) analysis of p-Fyn (Y530) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate (10ug) Lane2:U-87MG whole cell lysate (10ug) Lane3:HepG2 whole cell lysate (20ug) Lane4:C6 whole cell lysate (40ug) Lane5:BV2 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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