Caspase-8 (phospho-T341) polyclonal antibody
Product Specifications
Background
Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition) . Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Caspase-8; Caspase8; Caspase 8; CASP-8; Apoptotic cysteine protease; Apoptotic protease Mch-5; CAP4; FADD-homologous ICE/ced-3-like protease; FADD-like ICE; FLICE; ICE-like apoptotic protease 5; MORT1-associated ced-3 homolog; MACH; Caspase-8 subunit p18; Caspase-8 subunit p10; CASP8; MCH5
Swiss Prot
Q14790
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human Caspase-8 around the phosphorylation site of Threonine 341.
Conjugation
Unconjugated
Related Pathways
Regulation of Apoptosis, Necrotic Cell Death, Inhibition of Apoptosis, Death Receptor Signaling
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 38, 55 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-Caspase 8 (T341) polyclonal antibody detects endogenous levels of Caspase 8 protein only when phosphorylated at Thr341.
Applications Notes
Western blot (WB) analysis of p-Caspase-8 (T341) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate (40ug) Lane2:HEK293T treated with UV for 1h whole cell lysate (40ug) Lane3:HEK293T treated with UV for 6h whole cell lysate (40ug) Lane4:HEK293T treated with UV for 24h whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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