GFAP (phospho-Ser38) polyclonal antibody

Product Specifications

Background

Glial fibrillary acidic protein, or GFAP, is an intermediate filament (IF) protein belonging to the type III subclass of IF proteins. Like other IF proteins, GFAP is composed of an amino-terminal head domain, a central rod domain and a carboxy-terminal tail domain. GFAP is specifically found in astroglia, a cell type which is highly responsive to neurologic insults. Astrogliosis is found to be a result of mechanical trauma, AIDS dementia, prion infection and inflammatory demylination diseases, and is accompanied by an increase in GFAP expression. GFAP is an immunohistochemical marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Glial fibrillary acidic protein

Swiss Prot

P14136

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human GFAP around the phosphorylation site of Serine 38.

Conjugation

Unconjugated

Related Pathways

Neuronal and Glial Cell Marker Atlas

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 55 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-GFAP (Ser38) polyclonal antibody detects endogenous levels of GFAP protein when phosphorylated at Ser38.

Applications Notes

Western blot (WB) analysis of p-GFAP (Ser38) pAb at 1:500 dilution Lane1:The Brain tissue lysate of Rat (30ug) Lane2:The Brain tissue lysate of Mouse (40ug)