NPM (phospho-T199) polyclonal antibody

Product Specifications

Background

Nucleophosmin (NPM; also known as B23, numatrin or NO38) is an abundant phosphoprotein primarily found in nucleoli. It has been implicated in several distinct cellular functions, including assembly and transport of ribosomes, cytoplasmic/nuclear trafficking, regulation of DNA polymerase α activity, centrosome duplication and molecular chaperoning activities. The NPM gene is also known for its fusion with the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. The NPM portion contributes to transformation by providing a dimerization domain, which results in activation of the fused kinase.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Nucleophosmin; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Numatrin; NPM1; NPM

Swiss Prot

P06748

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human NPM around the phosphorylation site of Threonine 199.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 97% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 38 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-NPM (T199) polyclonal antibody detects endogenous levels of NPM protein only when phosphorylated at Thr199.

Applications Notes

Western blot (WB) analysis of p-NPM (T199) pAb at 1:500 dilution Lane1:H1792 whole cell lysate (40ug) Lane2:A549 whole cell lysate (40ug) Lane3:SGC7901 whole cell lysate (40ug) Lane4:EC9706 whole cell lysate (40ug) Lane5:PC12 whole cell lysate (40ug) Lane6:CT26 whole cell lysate (40ug) Lane7:HEK293T whole cell lysate (40ug)