MSK1 (phospho-Thr581) polyclonal antibody

MSK-1 is a mitogen and stress activated protein kinase-1 which belongs to the AGC family of kinases and is related in structure to the ribosomal p70 S6 kinase subfamily. MSK-1can be activated by ERK1/2 and SAPK2 /p38 MAP kinase. It is also known to be required for the phosphorylation of CREB, ATF1 H3 and HMG-14 in response to mitogen and stress. Similar to RSK, MSK-1 contains two kinase domains (N-term and a C-term) . Once phosphorylated on Thr581 and Ser360 by ERK1/2 and SAPK2/p38, MSK-1 autophosphorylate on at least 5 sites. Of these autophosphorylation sites Ser212 and Ser376 get phosphorylated by the C-terminal kinase domain of MSK-1 which is essential for the catalytic activity of the N-terminal kinase domain.

Ribosomal protein S6 kinase alpha-5; S6K-alpha-5; 90 kDa ribosomal protein S6 kinase 5; Nuclear mitogen- and stress-activated protein kinase 1; RSK-like protein kinase; RSKL; RPS6KA5; MSK1

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 96% (by SDS-PAGE) .

Western blot (WB) analysis of p-MSK1 (Thr581) polyclonal antibody at 1:500 or 1:2000 dilution Lane1:H9C2 treated with LPS (40ng/ml) for 45min whole cell lysate Lane2:H9C2 treated with LPS (40ng/ml) for 15min whole cell lysate Lane3:H9C2 treated with LPS (40ng/ml) for 5min whole cell lysate Lane4:L02 treated with LPS (40ng/ml) for 45min whole cell lysate Lane5:L02 treated with LPS (40ng/ml) for 15min whole cell lysate Lane6:L02 whole cell lysate Lane7:The Brain tissue lysate of Mouse (40ug)