Myc-tag (5D10) monoclonal antibody

Product Specifications

Background

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da) . It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

CAS Number

9007-83-4

Structure Composition

1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Myc, c Myc Epitope Tag, c Myc Tag, Myc Epitope Tag, Myc proto-oncogene protein, Transcription factor p64

Reactivity

Transfected

Immunogen

Synthetic peptide Myc-tag.

Conjugation

Unconjugated

Applications

Dilution

WB:1:5000~10000

Purity

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

The antibody detects over-expressed or recombinant proteins containing the Myc epitope tag.

Applications Notes

Western blot (WB) analysis of over-expressed Myc-tagged protein in HEK293T cell lysate, the antibody dilutions are 1:5000 (lane 1) and 1:10000 (lane 2) . Each lane was loaded with 10 μg of cell lysate.