HA-tag (4G3) monoclonal antibody
Product Specifications
Background
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
CAS Number
9007-83-4
Structure Composition
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
HA epitope tag; HA Tag; hemagglutinin
Reactivity
Transfected
Immunogen
Synthetic peptide HA-tag.
Conjugation
Unconjugated
Applications
WB IF IP
Dilution
WB: 1:1000~1:5000 IF: 1:1000 IP: 1:100
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
HA-tag (4G3) mAb detects over-expressed proteins containing the HA epitope tag.
Applications Notes
Western blot (WB) analysis of over-expressed HA-tagged protein in HEK293T cell lysate, the antibody dilutions are 1:1000 (lane 1) and 1:5000 (lane 2) . Each lane was loaded with 10 μg of cell lysate.
Host or Source
Mouse
Available Sizes
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