SPAG5 polyclonal antibody

Product Specifications

Background

SPAG5 (sperm associated antigen 5), also known as MAP126, hMAP126, DEEPEST or Astrin, is a 1,193 amino acid protein that is expressed at high levels in testis and at lower levels in liver, placenta, pancreas, colon and thymus. Localized specifically to spindle poles during prophase and throughout the spindle during metaphase and anaphase, SPAG5 is essential for proper spindle formation and is thought to be involved in the dynamic and functional regulation of mitotic spindles. Additionally, SPAG5 plays a role in sister chromatid cohesion and subsequent chromatid separation and, via its association with chromatids, is an important regulator of chromosome integrity. SPAG5 has a long stalk and a globular head domain through which if forms a homodimer that is characterized by aster-like structures. Upon DNA damage, SPAG5 may be phosphorylated by ATR or ATM.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Sperm-associated antigen 5; Astrin; Deepest; Mitotic spindle-associated protein p126; MAP126; SPAG5

Swiss Prot

Q96R06

Reactivity

Human

Immunogen

A synthetic peptide corresponding to residues in Human SPAG5

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 134, 150 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

SPAG5 polyclonal antibody detects endogenous levels of SPAG5 protein.

Applications Notes

Western blot (WB) analysis of SPAG5 pAb at 1:500 dilution Lane1:Myla2059 whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:HuT78 whole cell lysate (40ug)