EIF4E (T203) polyclonal antibody

Product Specifications

Background

EIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types. Several kinases are currently being investigated as potential regulators of eIF4E including PKC and/or the MAP kinase activated Mnk.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

EIF4E (phospho S209) ; eIF-4E; eIF4E; mRNA cap-binding protein; eIF-4F 25 kDa subunit; EIF4E; 1977; EIF4E; 133440; P06730; CBP; EIF4EL1; EIF4F; MGC111573

Swiss Prot

P06730

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 171-210 of Human eIF4E.

Conjugation

Unconjugated

Related Pathways

Insulin &Glucose Signaling

Applications

WB IHC IP

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:10~1:100

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 25 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

EIF4E (T203) polyclonal antibody detects endogenous levels of eIF4E protein.

Applications Notes

Western blot (WB) analysis of eIF4E (T203) pAb at 1:1000 dilution Lane1:Hela whole cell lysate (40ug) Lane2:SGC7901 whole cell lysate (40ug) Lane3:The Spleen tissue lysate of Rat (40ug) Lane4:3T3-L1 whole cell lysate (40ug) Lane5:BV2 whole cell lysate (40ug)