Nrf2 (L593) polyclonal antibody

Product Specifications

Background

Nuclear factor erythroid 2-related factor 2 (Nrf2) is a transcription factor which regulates the expression of many detoxification and antioxidant enzymes. Nrf2 can potentially play a significant role in adaptive responses to oxidative stress. Nrf2 belongs to the Cap N Collar (CNC-bZIP subfamily of basic /leucine zipper (bZIP) transcription factors.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Nuclear factor erythroid 2-related factor 2; NF-E2-related factor 2; NFE2-related factor 2; HEBP1; NFE2L2; NRF2

Swiss Prot

Q16236

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 560-621 of Human Nrf2.

Conjugation

Unconjugated

Related Pathways

AMPK Signaling Pathway

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 95 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Nrf2/NFE2L2 (L593) polyclonal antibody detects endogenous levels of Nrf2/NFE2L2 protein.

Applications Notes

Western blot (WB) analysis of Nrf2 (L593) pAb at 1:500 dilution Lane1:PC12 whole cell lysate (40ug) Lane2:Myla2059 whole cell lysate (40ug) Lane3:SGC7901 whole cell lysate (40ug) Lane4:A549 whole cell lysate (40ug)