PLC β3 (L532) polyclonal antibody
Product Specifications
Background
A total of eight mammalian PLC isozymes have been described (PLC β1, PLC β2, PLC β3, PLC β4, PLC γ1, PLC γ2, PLC δ1 and PLC δ2) with molecular weights ranging from 85 to 150 kDa. The γ-type enzymes are unique in that they contain SH2 and SH3 domains. Moreover, the two γ-type enzymes, but not the β and δ isozymes, are subject to activation by a number of protein tyrosine kinases which associate with their SH2 domains and induce their activation by phosphoryation. In contrast, activation of PLC β1, PLC β2 and PLC β3 is mediated by the α subunits of the Gq class of heterotrimeric G proteins and by certain βγ G protein subunits. The regulatory mechanisms for PLC δ1 and PLC δ2 are as yet not resolved.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase β-3; Phosphoinositide phospholipase C-β-3; Phospholipase C-β-3; PLC-β3; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3; Phosphoinositide phospholipase C-beta-3; Phospholipase C-beta-3; PLC-beta-3; PLCB3; PLCβ3
Swiss Prot
Q01970
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 500-550 of Human PLC β3.
Conjugation
Unconjugated
Related Pathways
Phosphoinosotode Signaling, Phospholipase Signaling
Applications
WB IHC IF
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 150 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
PLC β3 (L532) polyclonal antibody detects endogenous levels of PLC β3 protein.
Applications Notes
Western blot (WB) analysis of PLCB3 pAb at 1:500 dilution Lane1:HCT116 whole cell lysate (40ug) Lane2:Beas-2B whole cell lysate (40ug) Lane3:PMVEC whole cell lysate (40ug) Lane4:CT26 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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