PLC β3 (L532) polyclonal antibody

Product Specifications

Background

A total of eight mammalian PLC isozymes have been described (PLC β1, PLC β2, PLC β3, PLC β4, PLC γ1, PLC γ2, PLC δ1 and PLC δ2) with molecular weights ranging from 85 to 150 kDa. The γ-type enzymes are unique in that they contain SH2 and SH3 domains. Moreover, the two γ-type enzymes, but not the β and δ isozymes, are subject to activation by a number of protein tyrosine kinases which associate with their SH2 domains and induce their activation by phosphoryation. In contrast, activation of PLC β1, PLC β2 and PLC β3 is mediated by the α subunits of the Gq class of heterotrimeric G proteins and by certain βγ G protein subunits. The regulatory mechanisms for PLC δ1 and PLC δ2 are as yet not resolved.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase β-3; Phosphoinositide phospholipase C-β-3; Phospholipase C-β-3; PLC-β3; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3; Phosphoinositide phospholipase C-beta-3; Phospholipase C-beta-3; PLC-beta-3; PLCB3; PLCβ3

Swiss Prot

Q01970

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 500-550 of Human PLC β3.

Conjugation

Unconjugated

Related Pathways

Phosphoinosotode Signaling, Phospholipase Signaling

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 150 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

PLC β3 (L532) polyclonal antibody detects endogenous levels of PLC β3 protein.

Applications Notes

Western blot (WB) analysis of PLCB3 pAb at 1:500 dilution Lane1:HCT116 whole cell lysate (40ug) Lane2:Beas-2B whole cell lysate (40ug) Lane3:PMVEC whole cell lysate (40ug) Lane4:CT26 whole cell lysate (40ug)