Calpain 2 (K354) polyclonal antibody

Product Specifications

Background

Mu Calpain, and m calpain, also known as Calpain 2, are intracellular, calcium dependent cysteine proteases. Mu calpain has a micromolar sensitivity (thus the mu) as compared to the millimolar calcium sensitivity of m calpain. Both Calpains 1 and 2 are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca++ requirements that are suggested by their names.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Calpain-2 catalytic subunit; Calcium-activated neutral proteinase 2; CANP 2; Calpain M-type; Calpain large polypeptide L2; Calpain-2 large subunit; Millimolar-calpain; M-calpain; CANPL2; CAPN2

Swiss Prot

P17655

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 320-370 of Human Calpain 2.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 80 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Calpain 2 (K354) polyclonal antibody detects endogenous levels of Calpain-2 catalytic subunit protein.

Applications Notes

Western blot (WB) analysis of Calpain 2 (K354) pAb at 1:500 dilution Lane1:Beas-2B whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:PC3 whole cell lysate (40ug) Lane4:The Kidney tissue lysate of Mouse (40ug) Lane5:The Kidney tissue lysate of Rat (40ug)