Wee1 polyclonal antibody

Product Specifications

Background

Entry of all eukaryotic cells into mitosis is regulated by activation of cdc2 kinase. The critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Thr14. Phosphorylation at Tyr15 and Thr14 and inhibition of cdc2 is carried out by Wee1 and Myt1 protein kinases, while Tyr15 dephosphorylation and activation of cdc2 is carried out by the cdc25 phosphatase. Hyperphosphorylation and inactivation of Myt1 in mitosis suggests that one or more kinases activated at the G2/M transition negatively regulates Myt1 activity. Kinases shown to phosphorylate Myt1 include cdc2, p90RSK, Akt, and Plk1. Wee1 is inactivated upon mitotic entry by phosphorylation at Ser53 and Ser123 by Plk1 and cdc2, followed by beta-TrCP-mediated ubiquitination and degradation.

CAS Number

9007-83-4

Structure Composition

1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.3.

Product Name Alternative

Wee1-like protein kinase; WEE1hu; Wee1A kinase; WEE1

Swiss Prot

P30291

Reactivity

Human, Rat, Mouse

Immunogen

Synthetic peptide, corresponding to Human Wee1.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 80 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Wee1 polyclonal antibody detects endogenous levels of Wee1 protein.

Applications Notes

Western blot (WB) analysis of Wee1 polyclonal antibody at 1:500 dilution Lane1:Myla2059 whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (40ug) Lane3:Beas-2B whole cell lysate (40ug) Lane4:Hela whole cell lysate (40ug) Lane5:HuT78 whole cell lysate (40ug) Lane6:3T3-L1 whole cell lysate (40ug) Lane7:C6 whole cell lysate (40ug)