SREBP-1 (phospho-S439) polyclonal antibody
Product Specifications
Background
Transcription of the LDL receptor gene is controlled by a ten base pair sequence in the 5' flanking region, designated sterol regulatory element 1 (SRE-1) . When cellular sterol stores are depleted, the element is activated, the gene is transcribed and the cellular uptake of LDL increases. SREBP-1 (also designated ADD1, for adipocyte determination and differentiation factor) is synthesized as a precursor that is attached to the nuclear envelope and endoplasmic reticulum. In sterol-depleted cells, the membrane-bound precursor is cleaved to generate a soluble NH2-terminal fragment that translocates to the nucleus to activate transcription. Sterols inhibit the cleavage of SREBP-1.
CAS Number
9007-83-4
Structure Composition
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.3.
Product Name Alternative
Sterol regulatory element-binding protein 1; SREBP-1; Class D basic helix-loop-helix protein 1; bHLHd1; Sterol regulatory element-binding transcription factor 1; Processed sterol regulatory element-binding protein 1; SREBF1; BHLHD1; SREBP1
Swiss Prot
P36956
Reactivity
Human, Rat, Mouse
Immunogen
Synthetic phosphopeptide derived from human SREBP-1 around the phosphorylation site of Serine 439
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 121 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
SREBP-1 (phospho-S439) polyclonal antibody detects endogenous levels of SREBP-1 protein only when phosphorylated at Ser439.
Applications Notes
Western blot (WB) analysis of SREBP-1 (phospho-S439) polyclonal antibody at 1:500 dilution Lane1:Myla2059 whole cell lysate (40ug) Lane2:RAW264.7 whole cell lysate (40ug) Lane3:PC12 whole cell lysate (40ug) Lane4:HEK293T whole cell lysate (40ug) Lane5:HepG2 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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