Cdk9 (phospho-T186) polyclonal antibody
Product Specifications
Background
A family of proteins designated cyclin dependent kinases (Cdks) are critical regulators of cell cycle progression. Cdk family members, including Cdc2 p34, Cdk1–9, PISSLRE, KKIALRE, PITSLRE and PCTAIRE 1–3 are constitutively expressed throughout the cell cycle. Cdc2 p34 activity peaks during mitosis and Cdk2 activity rises in late G1 or early S phase. Cdk4 and Cdk6 are critically involved in G1 to S phase progression. The functions of Cdk3, Cdk5b, PISSLRE, KKIALRE and PCTAIRE 1–3 are less well defined. Cdk9 (also designated PITALRE) has been shown to specifically phosphorylate the retinoblastoma protein. The more recently cloned Drosophila protein, P-TEFb, is thought to be the homolog of mammalian PITALRE. P-TEFb has been shown to be required for HIV Tat transcriptional activation.
CAS Number
9007-83-4
Structure Composition
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.3.
Product Name Alternative
Cyclin-dependent kinase 9; C-2K; Cell division cycle 2-like protein kinase 4; Cell division protein kinase 9; Serine/threonine-protein kinase PITALRE; Tat-associated kinase complex catalytic subunit; CDC2L4; TAK
Swiss Prot
P50750
Reactivity
Human, Rat, Mouse
Immunogen
Synthetic phosphopeptide derived from human Cdk9 around the phosphorylation site of Threonine 186
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 42, 55 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Cdk9 (phospho-T186) polyclonal antibody detects endogenous levels of Cdk9 protein only when phosphorylated at Thr186.
Applications Notes
Western blot (WB) analysis of Cdk9 (phospho-T186) polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (40ug) Lane3:The Spleen tissue lysate of Mouse (40ug) Lane4:C6 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
Curated Selection
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