IRS-1 (Phospho-Ser794) polyclonal antibody
Product Specifications
Background
Insulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase. IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain containing proteins that mediate the metabolic and growth-promoting functions of insulin. IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser307 of IRS-1 is phosphorylated by JNK and IKK while Ser789 is phosphorylated by SIK-2, a member of the AMPK family. The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 and Ser636/639, respectively. Phosphorylation of IRS-1 at Ser1101 is mediated by PKCθ and results in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity.
CAS Number
9007-83-4
Structure Composition
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.3.
Product Name Alternative
Insulin receptor substrate 1; IRS-1; IRS1
Swiss Prot
P35568
Reactivity
Human
Immunogen
Synthetic phosphopeptide derived from human IRS-1 around the phosphorylation site of Serine 794.
Conjugation
Unconjugated
Related Pathways
Translational Contral elF4 and p70 S6 Kinase, Insulin &Glucose Signaling
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 180 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
IRS-1 (Phospho-Ser794) polyclonal antibody detects endogenous levels of IRS-1 protein only when phosphorylated at Ser794.
Applications Notes
Western blot (WB) analysis of IRS-1 (Phospho-Ser794) polyclonal antibody at 1:500 dilution Lane1:A375 whole cell lysate (40ug) Lane2:A2780 whole cell lysate (40ug) Lane3:HepG2 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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