IRS-1 (Phospho-Ser794) polyclonal antibody

Product Specifications

Background

Insulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase. IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain containing proteins that mediate the metabolic and growth-promoting functions of insulin. IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser307 of IRS-1 is phosphorylated by JNK and IKK while Ser789 is phosphorylated by SIK-2, a member of the AMPK family. The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 and Ser636/639, respectively. Phosphorylation of IRS-1 at Ser1101 is mediated by PKCθ and results in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity.

CAS Number

9007-83-4

Structure Composition

1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.3.

Product Name Alternative

Insulin receptor substrate 1; IRS-1; IRS1

Swiss Prot

P35568

Reactivity

Human

Immunogen

Synthetic phosphopeptide derived from human IRS-1 around the phosphorylation site of Serine 794.

Conjugation

Unconjugated

Related Pathways

Translational Contral elF4 and p70 S6 Kinase, Insulin &Glucose Signaling

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 180 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

IRS-1 (Phospho-Ser794) polyclonal antibody detects endogenous levels of IRS-1 protein only when phosphorylated at Ser794.

Applications Notes

Western blot (WB) analysis of IRS-1 (Phospho-Ser794) polyclonal antibody at 1:500 dilution Lane1:A375 whole cell lysate (40ug) Lane2:A2780 whole cell lysate (40ug) Lane3:HepG2 whole cell lysate (40ug)