FGFR4 (phospho-Y642) polyclonal antibody

Product Specifications

Background

Acidic and basic fibroblast growth factors (FGFs) are members of a family of multifunctional polypeptide growth factors that stimulate proliferation of cells of mesenchymal, epithelial and neuroectodermal origin. Like other growth factors, FGFs act by binding and activating specific cell surface receptors. These include the Flg receptor or FGFR-1, the Bek receptor or FGFR-2, FGFR-3, FGFR-4, FGFR-5 and FGFR-6. These receptors usually contain an extracellular ligandbinding region containing three immunoglobulin-like domains, a transmembrane domain and a cytoplasmic tyrosine kinase domain. The gene encoding human FGFR-4, unlike the other FGFR genes, is alternatively spliced to produce only one isoform. It is expressed in fetal adrenal, lung, kidney, liver, pancreas, intestine, striated muscle and spleen tissues. FGFR-4 is also overexpressed in breast cancers and, subsequently, is a potential target for drug therapy.

CAS Number

9007-83-4

Structure Composition

1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.3.

Product Name Alternative

Fibroblast growth factor receptor 4 ; FGFR-4; CD334; FGFR4; JTK2; TKF

Swiss Prot

P22455

Reactivity

Human

Immunogen

Synthetic phosphopeptide derived from human FGFR4 around the phosphorylation site of Tyrosine642.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 100 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

FGFR4 (phospho-Y642) polyclonal antibody detects endogenous levels of FGFR4 protein only when phosphorylated at Try642 .

Applications Notes

Western blot (WB) analysis of FGFR4 (phospho-Y642) polyclonal antibody at 1:500 dilution Lane1:HepG2 whole cell lysate (40ug) Lane2:SGC7901 whole cell lysate (40ug) Lane3:Panc1 whole cell lysate (40ug)