TUBA1A (phospho-Y272) polyclonal antibody

Tubulin is a major cytoskeleton component that has five distinct forms, designated α, β, γ, δ and ε Tubulin. α and β Tubulins form heterodimers which multimerize to form a microtubule filament. Multiple β Tubulin isoforms (β1, β2, β3, β4, β5, β6 and β8) have been characterized and are expressed in mammalian tissues. β1 and β4 are present throughout the cytosol, β2 is present in the nuclei and nucleoplasm, and β3 is a neuron-specific cytoskeletal protein. γ Tubulin forms the gammasome, which is required for nucleating microtubule filaments at the centrosome. Both δ Tubulin and ε Tubulin are associated with the centrosome. δ Tubulin is a homolog of the Chlamydomonas δ Tubulin Uni3 and is found in association with the centrioles, whereas ε Tubulin localizes to the pericentriolar material. ε Tubulin exhibits a cellcycle-specific pattern of localization, first associating with only the older of the centrosomes in a newly duplicated pair and later associating with both centrosomes.

Tubulin alpha-1A chain; Alpha-tubulin 3; Tubulin B-alpha-1; Tubulin alpha-3 chain; Detyrosinated tubulin alpha-1A chain; TUBA1A; TUBA3; Tubulin alpha-1C chain; Alpha-tubulin 6; Tubulin alpha-6 chain; Detyrosinated tubulin alpha-1C chain; TUBA1C; TUBA6; Tubulin alpha-3E chain; Alpha-tubulin 3E; Detyrosinated tubulin alpha-3E chain; TUBA3E; Tubulin alpha-4A chain; Alpha-tubulin 1; Testis-specific alpha-tubulin; Tubulin H2-alpha; Tubulin alpha-1 chain; TUBA4A; TUBA1

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Western blot (WB) analysis of TUBA1A (phospho-Y272) polyclonal antibody at 1:500 dilution Lane1:U-87MG whole cell lysate (40ug) Lane2:A375 whole cell lysate (40ug) Lane3:Hela whole cell lysate (40ug) Lane4:A2780 whole cell lysate (40ug) Lane5:EC9706 whole cell lysate (40ug) Lane6:C6 whole cell lysate (40ug) Lane7:CT26 whole cell lysate (40ug)