RAF1 (phospho-S289) polyclonal antibody

Product Specifications

Background

Raf-1 is an ubiquitously expressed cytoplasmic protein with intrinsic serine/ threonine kinase activity. Raf-1, or c-Raf, is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein is associated with cellular transformation and mitogenesis. Raf-1 is normally suppressed by its regulatory N-terminal domain. Raf-1 is activated in response to a variety of tyrosine kinase receptors as well as in response to pp60v-Src expression. Specifically, Raf-1 is phosphorylated in the catalytic domain at Ser 338 and, to a lesser extent, Ser 339. This phosphorylation requires the co-activation of PI 3-kinase and the Ras signaling pathway. Raf-1 is also phosphorylated on Tyr 340 and 341, which induces the phosphorylation of MEK. Phosphorylation of Ser 621 is essential for the catalytic activity of Raf-1 and downregulation by c-AMP-dependent protein kinase A (PKA) . PKA also phosphorylates Raf-1 on Ser 43 and Ser 259. PKA phosphorylation of Ser 259 inhibits Raf-1 and decreases the phosphorylation necessary for Raf-1 activation at Ser 338.

CAS Number

9007-83-4

Structure Composition

1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.3.

Product Name Alternative

RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1; RAF1; RAF

Swiss Prot

P04049

Reactivity

Human, Mouse

Immunogen

Synthetic phosphopeptide derived from human RAF1 around the phosphorylation site of Serine289.

Conjugation

Unconjugated

Related Pathways

Inhibition of Apoptosis, G Protein-coupled Receptors Signaling to MAPK Erk, hippo Signaling, MAPK Erk in Growth and Differentiation Pathway

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 75 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

RAF1 (phospho-S289) polyclonal antibody detects endogenous levels of RAF1 protein only when phosphorylated at Ser289 .

Applications Notes

Western blot (WB) analysis of RAF1 (phospho-S289) polyclonal antibody at 1:500 dilution Lane1:AML-12 whole cell lysate (40ug) Lane2:CT26 whole cell lysate (40ug) Lane3:PC12 whole cell lysate (40ug) Lane4:HEK293T whole cell lysate (40ug) Lane5:A549 whole cell lysate (40ug)