NR3C1 (phospho-S203) pAb

Product Specifications

Background

The glucocorticoid receptor (GR) is an ubiquitously expressed transcription factor that mediates the effects of glucocorticoids. The most abundant isoform is GR α. GR induces or represses the expression of genes in response to glucocorticoids, mediating such processes as apoptosis and cell growth and differentiation. A significant class of genes suppressed by GR is controlled by the transcription factor AP-1. GR has also been shown to be the limiting factor in the induction of gene expression by glucocorticoids. It has been revealed that GR forms a complex with HSP 90, rendering the nonligand bound receptor transcriptionally inactive. More importantly, mutant GRs lacking the signaling domain remain constitutively active.

Structure Composition

1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.3.

Product Name Alternative

Glucocorticoid receptor; GR; Nuclear receptor subfamily 3 group C member 1; NR3C1; GRL

Swiss Prot

P04150

Reactivity

Human, Rat, Mouse

Immunogen

Synthetic phosphopeptide derived from human NR3C1 around the phosphorylation site of Serine203.

Conjugation

Unconjugated

Related Pathways

Nuclear Receptor Signaling, Nuclear Receptor Signaling

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 95 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

NR3C1 (Phospho-S203) polyclonal antibody detects endogenous levels of NR3C1 protein only when phosphorylated at Ser203 .

Applications Notes

Western blot (WB) analysis of NR3C1 (Phospho-S203) polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate (40ug) Lane2:A375 whole cell lysate (40ug) Lane3:L02 whole cell lysate (40ug) Lane4:AML-12 whole cell lysate (40ug) Lane5:PMVEC whole cell lysate (40ug)