CASP9 (phospho-S144) polyclonal antibody

Product Specifications

Background

Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family. Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf-1. Apaf-1 mediated activation of caspase-9 involves intrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit. Another cleavage occurs at Asp330 producing a p37 subunit that can serve to amplify the apoptotic response. Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis.

CAS Number

9007-83-4

Structure Composition

1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.3.

Product Name Alternative

Caspase-9; CASP-9; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3; ICE-like apoptotic protease 6; ICE-LAP6; Caspase-9 subunit p35; Caspase-9 subunit p10; CASP9; MCH6

Swiss Prot

P55211

Reactivity

Human

Immunogen

Synthetic phosphopeptide derived from human CASP9 around the phosphorylation site of Serine 144.

Conjugation

Unconjugated

Related Pathways

Regulation of Apoptosis, Inhibition of Apoptosis, Mitochondrial Control of Apoptosis, Death Receptor Signaling

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 47 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

CASP9 (Phospho-S144) polyclonal antibody detects endogenous levels of CASP9 protein only when phosphorylated at Ser144.

Applications Notes

Western blot (WB) analysis of CASP9 (Phospho-S144) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate (40ug) Lane2:HepG2 whole cell lysate (40ug) Lane3:A375 whole cell lysate (40ug)