CASP9 (phospho-S144) polyclonal antibody
Product Specifications
Background
Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family. Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf-1. Apaf-1 mediated activation of caspase-9 involves intrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit. Another cleavage occurs at Asp330 producing a p37 subunit that can serve to amplify the apoptotic response. Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis.
CAS Number
9007-83-4
Structure Composition
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.3.
Product Name Alternative
Caspase-9; CASP-9; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3; ICE-like apoptotic protease 6; ICE-LAP6; Caspase-9 subunit p35; Caspase-9 subunit p10; CASP9; MCH6
Swiss Prot
P55211
Reactivity
Human
Immunogen
Synthetic phosphopeptide derived from human CASP9 around the phosphorylation site of Serine 144.
Conjugation
Unconjugated
Related Pathways
Regulation of Apoptosis, Inhibition of Apoptosis, Mitochondrial Control of Apoptosis, Death Receptor Signaling
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 47 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
CASP9 (Phospho-S144) polyclonal antibody detects endogenous levels of CASP9 protein only when phosphorylated at Ser144.
Applications Notes
Western blot (WB) analysis of CASP9 (Phospho-S144) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate (40ug) Lane2:HepG2 whole cell lysate (40ug) Lane3:A375 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
