PKD1 (phospho-S205) polyclonal antibody
Product Specifications
Background
Activation of PKC is one of the earliest events in a cascade leading to a variety of cellular responses such as secretion, gene expression, proliferation and muscle contraction. Protein kinase D (PKD), also called PKCμ, is a serine/threonine kinase whose activation is dependent on the phosphorylation of two activation loop sites, Ser744 and Ser748, via a PKC-dependent signaling pathway. In addition to the two activation loop sites, the carboxy-terminal Ser916 has been identified as an autophosphorylation site for PKD/PKCμ. Phosphorylation at Ser916 correlates with PKD/PKCμ catalytic activity.
CAS Number
9007-83-4
Structure Composition
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.3.
Product Name Alternative
Serine/threonine-protein kinase D1; Protein kinase C mu type; Protein kinase D; nPKC-D1; nPKC-mu; PRKD1; PKD; PKD1; PRKCM
Swiss Prot
Q15139
Reactivity
Human, Rat, Mouse
Immunogen
Synthetic phosphopeptide derived from human PRKD1 around the phosphorylation site of Serine 205.
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 115 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
PRKD1 (Phospho-S205) polyclonal antibody detects endogenous levels of PRKD1 protein only when phosphorylated at Ser205.
Applications Notes
Western blot (WB) analysis of PRKD1 (Phospho-S205) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:HEK293T treated with PMA (50ng/ml,10 minutes) whole cell lysate Lane3:HEK293T treated with PMA (50ng/ml,30 minutes) whole cell lysate Lane4:H9C2 whole cell lysate Lane5:3T3-L1 whole cell lysate
Host or Source
Rabbit
Available Sizes
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