PGR (phospho-S400) polyclonal antibody
Product Specifications
Background
Human progesterone receptor (PR) is expressed as two forms: the full length PR-B and the short form PR-A. PR-A lacks the first 164 amino acid residues of PR-B. Both PR-A and PR-B are ligand activated, but differ in their relative ability to activate target gene transcription. The activity of PR is regulated by phosphorylation; at least seven serine residues are phosphorylated in its amino-terminal domain. Three sites (Ser81, Ser102, and Ser162) are unique to full length PR-B, while other sites (Ser190, Ser294, Ser345, and Ser400) are shared by both isoforms. Phosphorylation of PR-B at Ser190 (equivalent to Ser26 of PR-A) is catalyzed by CDK2. Mutation of Ser190 results in decreased activity of PR, suggesting that the phosphorylation at Ser190 may be critical to its biological function.
CAS Number
9007-83-4
Structure Composition
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.3.
Product Name Alternative
Progesterone receptor; PR; Nuclear receptor subfamily 3 group C member 3; PGR; NR3C3
Swiss Prot
P06401
Reactivity
Human, Rat, Mouse
Immunogen
Synthetic phosphopeptide derived from human PGR around the phosphorylation site of Serine 400.
Conjugation
Unconjugated
Related Pathways
Nuclear Receptor Signaling
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 98 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
PGR (Phospho-S400) polyclonal antibody detects endogenous levels of PGR protein only when phosphorylated at Ser400.
Applications Notes
Western blot (WB) analysis of PGR (Phospho-S400) polyclonal antibody at 1:500 dilution Lane1:The Testis tissue lysate of Mouse (40ug) Lane2:The Ovary tissue lysate of Rat (40ug) Lane3:Hela whole cell lysate (30ug)
Host or Source
Rabbit
Available Sizes
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