Skp1 p19 (P72) polyclonal antibody

Cyclin A accumulates prior to cyclin B in the cell cycle, appears to be involved in control of S phase and has been shown to associate with cyclin dependent kinase-2 (Cdk2) . In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcription factors DP-1 and E2F and retinoblastoma protein p110. Two cyclin A-Cdk2 complex binding proteins, Skp1 p19 and Skp2 p45, have been described. Although the Skps (S phase kinase-associated proteins) associate with the active cyclin A-Cdk2 complex, they do not exhibit any regulatory effects on the complex. Abolition of Skp2 p45 function by either microinjection of anti-p45 antibodies or addition of antisense oligonucleotides prevents entry into S phase of both normal and transformed cells.

P19A; RNA polymerase II elongation factor-like protein; Organ of Corti protein 2; OCP-2; OCP-II; Transcription elongation factor B; SIII; SKP1; EMC19; OCP2; SKP1A; TCEB1L

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Western blot (WB) analysis of SKP1 pAb at 1:500 dilution Lane1:MCF-7 whole cell lysate (40ug) Lane2:A2780 whole cell lysate (40ug) Lane3:Hela whole cell lysate (40ug)