IKKα/β (D173) polyclonal antibody
Product Specifications
Background
The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins. Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB. The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit. Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
I kappa-B kinase alpha; IkBKA; IKK-alpha; IKK-A; IkappaB kinase; EC 2.7.11.10; I-kappa-B kinase 1; IKK1; Conserved helix-loop-helix ubiquitous kinase; Nuclear factor NF-kappa-B inhibitor kinase alpha; NFKBIKA; CHUK; IKKA; TCF16
Swiss Prot
O15111/O14920
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 151-200 of Human IKKα
Conjugation
Unconjugated
Related Pathways
NF-kB Signaling, PI3K AKT signaling, Immune Cell Signaling, STING Signaling, Inhibition of Apoptosis
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 85, 97 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
IKKα/β (D173) polyclonal antibody detects endogenous levels of IKKα/β protein.
Applications Notes
Western blot (WB) analysis of IKKα/β (D173) pAb at 1:500 dilution Lane1:MEF whole cell lysate (40ug) Lane2:H9C2 whole cell lysate (40ug) Lane3:Panc1 whole cell lysate (40ug) Lane4:PC3 whole cell lysate (40ug) Lane5:Jurkat whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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