GLK polyclonal antibody
Product Specifications
Background
Several mammalian kinases have been identified which exhibit sequence similarity to the Saccharomyces cerevisiae serine/threonine kinase STE20. STE20 is involved in relaying signals from G-protein coupled receptors to cytosolic MAP kinase cascades, and it lies upstream of a MAP kinase kinase kinase. Mammalian STE20-like kinases include GLK, KHS, NIK, YSK1, HPK1, Krs-1, Krs-2 and human GC kinase. GLK (for GC-like kinase) is an 885 amino acid protein that shares a high degree of homology with GC kinase and HPK1. Like many of the STE20-like kinases, GLK specifically activates the JNK pathway. Epistasis studies with dominant negative mutants of MEKK1 suggest that GLK functions upstream of MEKK1 in the JNK signaling pathway.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Mitogen-activated protein kinase kinase kinase kinase 3; MAPK/ERK kinase kinase kinase 3; MEK kinase kinase 3; Germinal center kinase related protein kinase; MEKKK 3; MAP4K3
Swiss Prot
Q8IVH8
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant full length Human GLK.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB: 1:500~1:2000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 110kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
GLK polyclonal antibody detects endogenous levels of GLK protein.
Applications Notes
Western blot analysis of extracts of various cell lines, using MAP4K3 antibody.
Host or Source
Rabbit
Available Sizes
Curated Selection
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