PI3K p110δ polyclonal antibody
Product Specifications
Background
Phosphatidylinositol 3-kinase (PI 3-kinase) is composed of p85 and p110 subunits. p85 lacks PI 3-kinase activity and acts as an adapter, coupling p110 to activated protein tyrosine kinase. Two forms of p85 have been described (p85α and p85β), each possessing one SH3 and two SH2 domains. Various p110 isoforms have been identified. p110α and p110β interact with p85α, and p110α has also been shown to interact with p85β in vitro. p110δ expression is restricted to white blood cells. It has been shown to bind p85α and β, but it apparently does not phosphorylate these subunits. p110δ seems to have the capacity to autophosphorylate. p110γ does not interact with the p85 subunits. It has been shown to be activated by α and βγ heterotrimeric G proteins.
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform; PI3-kinase subunit delta; PI3K-delta; PI3Kdelta; PtdIns-3-kinase subunit delta; Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit delta; PtdIns-3-kinase subunit p110-delta; p110delta; PIK3CD; PI3Kp110δ; PI3K-p110δ; PI3K δ; PI3Kδ; PI3K-δ
Swiss Prot
O00329
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to residues in Human PI3K p110δ.
Conjugation
Unconjugated
Related Pathways
Phosphoinosotode Signaling, Protein Kinase C Signaling, SAPK JNK Signaling Cascades, Inhibition of Apoptosis, G Protein-coupled Receptors Signaling to MAPK Erk, Adherens Junction signaling, Mitochondrial Control of Apoptosis
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 110 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
PI3K p110δ polyclonal antibody detects endogenous levels of PI3K p110δ protein.
Applications Notes
Western blot (WB) analysis of PIK3CD pAb at 1:500 dilution Lane1:The Testis tissue lysate of Mouse (40ug) Lane2:RAW264.7 whole cell lysate (40ug) Lane3:Myla2059 whole cell lysate (40ug) Lane4:H1792 whole cell lysate (40ug) Lane5:The Thymus tissue lysate of Rat (40ug) Lane6:SK-OVCAR3 whole cell lysate (40ug) Lane7:A549 whole cell lysate (40ug)
Host or Source
Rabbit
CAS Number
9007-83-4
Available Sizes
Curated Selection
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