DDDDK-tag (1A8) monoclonal antibody-HRP
Product Specifications
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.
CAS Number
9007-83-4
Structure Composition
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
DDDDK epitope tag; DYKDDDDK epitope tag; DYKDDDDK tag; ECS epitope tag, ECS tag; Enterokinase Cleavage Site epitope tag; Enterokinase Cleavage Site tag; DDDDK
Reactivity
Transfected
Immunogen
Synthetic peptide DDDDK-tag.
Conjugation
HRP
Applications
WB
Dilution
WB:1:1000~1:10000
Purity
Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to horseradish peroxidase. The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
DDDDK-tag (1A8) mAb-HRP detects over-expressed or recombinant proteins containing the DDDDK epitope tag.
Applications Notes
Western blot (WB) analysis of DDDDK-tag Mouse Antibody at 1:2000 dilution Lane1:HEK293T whole cell lysate , transfected with HA-DDDDK-p62 48h (3uL) Lane2:HEK293T whole cell lysate (40ug)
Host or Source
Mouse
Available Sizes
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
BNC940304-100 1x 100 µL
CD106 / VCAM1 (B-K9), CF594 conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC740204-100 1x 100 µL
Complement C4d (C4D204), CF740 conjugate, 0.1mg/mL
Sign In for Pricing
View Details 