DDDDK-tag (1A8) monoclonal antibody
Product Specifications
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.
CAS Number
9007-83-4
Structure Composition
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
DDDDK epitope tag; DYKDDDDK epitope tag; DYKDDDDK tag; ECS epitope tag, ECS tag; Enterokinase Cleavage Site epitope tag; Enterokinase Cleavage Site tag; DDDDK
Reactivity
Transfected
Immunogen
Synthetic peptide DDDDK-tag.
Conjugation
Unconjugated
Applications
WB IP IF
Dilution
WB: 1:5000~1:50000 IP: 1:100~1:1000 IF: 1:100~1:1000
Purity
The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
DDDDK-tag (1A8) mAb detects over-expressed or recombinant proteins containing the DDDDK epitope tag.
Applications Notes
Western blot (WB) analysis of DDDDK-tag (1A8) mAb at 1:10000/1:20000/1:30000 dillution Lane1:HEK293T whole cell lysate , untransfected Lane2:HEK293T whole cell lysate , transfected with pcDNA3.1-HA-DDDDK-p62
Host or Source
Mouse
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