MT-ND3 polyclonal antibody

Product Specifications

Background

NADH:ubiquinone oxidoreductase (complex I) is an extremely complicated multiprotein complex located in the inner mitochondrial membrane. Human complex I is important for energy metabolism because its main function is to transport electrons from NADH to ubiquinone, which is accompanied by trans-location of protons from the mitochondrial matrix to the intermembrane space. Human complex I appears to consist of 41 subunits. A small number of complex I subunits are the products of mitochondrial genes (subunits 1-7), while the remainder are nuclear encoded and imported from the cytoplasm. NADH dehydrogenase subunit 3 (ND3) localizes to the hydrophobic protein fragment of complex I. Mutations in the gene encodiing for ND3 may be associated with Parkinson disease.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

NADH-ubiquinone oxidoreductase chain 3 ; EC=1.6.5.3; NADH dehydrogenase subunit 3; MT-ND3; MTND3; NADH3; ND3

Swiss Prot

P03897

Reactivity

Human

Immunogen

A synthetic peptide corresponding to residues in Human MT-ND3.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 13 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

MT-ND3 polyclonal antibody detects endogenous levels of MT-ND3 protein.

Applications Notes

Western blot (WB) analysis of MT-ND3 pAb at 1:500 dilution Lane1:HepG2 whole cell lysate (40ug) Lane2:A549 whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:SGC7901 whole cell lysate (40ug)