14-3-3 θ/YWHAQ (phospho-S232) polyclonal antibody

Product Specifications

Background

Protein interactions with 14-3-3 show distinct preferences for it’s different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein. 14-3-3 tau/theta is primarily expressed in T-cells although it occurs at lower levels in the brain, heart, pancreas placenta and kidneys. It plays a role in cell cycle progression via interaction with p27 (Kip1) . Along with COPI, the theta isoform binds the GB1 RSR sequence involved in protein trafficking. The isoform forms a complex with Hsp60 and cellular prion protein which may be involved in prion diseases. Elevated levels of 14-3-3 tau/theta are observed in amyotrophic lateral sclerosis. 14-3-3 tau/theta also binds BCR/Abl, bacterial effector protein Tir, yes-associated protein and the FSH receptor.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

14-3-3 protein theta; 14-3-3 protein T-cell; 14-3-3 protein tau; Protein HS1; YWHAQ

Swiss Prot

P27348

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human YWHAQ around the phosphorylation site of Serine 232.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 28 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-YWHAQ (S232) polyclonal antibody detects endogenous levels of YWHAQ protein only when phosphorylated at Ser232.

Applications Notes

Western blot (WB) analysis of p-YWHAQ (S232) polyclonal antibody at 1:500 dillution Lane1: Hela whole cell lysate Lane2: Hela treated with LPS (100ng/ml, 30min) whole cell lysate Lane3: C6 whole cell lysate Lane4: C6 treated with EGF (0.1ng/ml, 30min) whole cell lysate Lane5: BV2 whole cell lysate Lane6: BV2 treated with LPS (100ng/ml, 30min) whole cell lysate