Smad2/3 (Phospho-T8) polyclonal antibody

Product Specifications

Background

Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, and 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7. Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2; SMAD2; MADH2; MADR2; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3; SMAD3; MADH3

Swiss Prot

Q15796/P84022

Reactivity

Human, Rat, Mouse

Immunogen

Synthetic phosphopeptide derived from human Smad2/3 around the phosphorylation site of Threonine 8.

Conjugation

Unconjugated

Related Pathways

Tumor Angiogenesis, Cell Cycle Control G1 S Checkpoint, Contribution of Soluble Factors to EMT, ESC Pluripotency and Differentiation Signaling Pathway, hippo Signaling, hippo Signaling

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 52 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Smad2/3 (Phospho-T8) polyclonal antibody detects endogenous levels of Smad2/3 protein only when phosphorylated at Thr8.

Applications Notes

Western blot (WB) analysis of Smad2/3 (Phospho-T8) polyclonal antibody at 1:500 dilution Lane1:C6 whole cell lysate (40ug) Lane2:MEF whole cell lysate (40ug) Lane3:A2780 whole cell lysate (40ug) Lane4:A549 whole cell lysate (40ug) Lane5:A375 whole cell lysate (40ug) Lane6:MCF-7 whole cell lysate (30ug) Lane7:Hela whole cell lysate (30ug)