ZC3HC1 (Phospho-S354) polyclonal antibody

Product Specifications

Background

NIPA (nuclear interaction partner of ALK) is an F-box-containing protein that is an essential component of the SCF-type E3 ligase (SCFNIPA) complex, a complex that controls the completion of S-phase and mitotic entry . This control is mediated by the ubiquitination and subsequent degradation of cell cycle regulatory proteins, whose oscillation of protein levels is required for proper cell cycle progression. Expression levels of NIPA are low in G0/G1 phases and upregulated in S and G2/M phases. The SCFNIPA complex targets nuclear cyclin B1 for ubiquitination in interphase, whereas phosphorylation of NIPA in late G2 phase and mitosis inactivates the complex to allow for accumulation of cyclin B1. NIPA may have an anti-apoptotic role in NPM-ALK-mediated signaling events.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Product Name Alternative

Nuclear-interacting partner of ALK; Nuclear-interacting partner of anaplastic lymphoma kinase; hNIPA; Zinc finger C3HC-type protein 1; ZC3HC1; NIPA; HSPC216

Swiss Prot

Q86WB0

Reactivity

Human, Mouse

Immunogen

Synthetic phosphopeptide derived from human ZC3HC1 around the phosphorylation site of Serine 354.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 65kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

ZC3HC1 (Phospho-S354) polyclonal antibody detects endogenous levels of ZC3HC1 protein only when phosphorylated at Ser354.

Applications Notes

Western blot (WB) analysis of ZC3HC1 (Phospho-S354) polyclonal antibody at 1:500 dilution Lane1:K562 whole cell lysate (40ug) Lane2:A2780 whole cell lysate (40ug) Lane3:AML-12 whole cell lysate (40ug) Lane4:Jurkat whole cell lysate (40ug)