DAB1 (Phospho-Y220) polyclonal antibody
Product Specifications
Background
Dab1, a homolog of the Drosophila disabled protein, is an adaptor protein involved in neural development. This cytoplasmic protein is tyrosine-phosphorylated during rapid expansion of the developing nervous system, and it is thought to interact with other proteins via a domain similar to the PTB domains of the Shc family. Dab1 has been shown to interact with the SH2 domains of Src, Fyn and Abl. Mutations in Dab1 result in widespread abnormalities in the brain, similar to those seen in Reelin mutants. Reelin is a secreted protein thought to play a role in directing migrating neurons. Evidence suggests that Dab1 functions downstream of Reelin in a signaling pathway involved in positioning cells in the developing brain. Dab2 (also designated DOC-2) is a mitogen-responsive phosphoprotein that binds the SH3 domain of GRB2, and it is thought to be a negative regulator of growth.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
Product Name Alternative
Disabled homolog 1; DAB1
Swiss Prot
O75553
Reactivity
Rat, Mouse
Immunogen
Synthetic phosphopeptide derived from human DAB1 around the phosphorylation site of Tyrosine 220.
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 64 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
DAB1 (Phospho-Y220) polyclonal antibody detects endogenous levels of DAB1 protein only when phosphorylated at Tyr220.
Applications Notes
Western blot (WB) analysis of DAB1 (Phospho-Y220) polyclonal antibody at 1:500 dilution Lane1:The Brain tissue lysate of Mouse (40ug) Lane2:The Brain tissue lysate of Rat (40ug)
Host or Source
Rabbit
Available Sizes
Curated Selection
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