Integrin β1 (Y783) polyclonal antibody

Product Specifications

Background

Integrins are heterodimers composed of noncovalently associated transmembrane α and β subunits. The 16 α and 8 β subunits heterodimerize to produce more than 20 different receptors. Most integrin receptors bind ligands that are components of the extracellular matrix, including fibronectin, collagen and vitronectin. Certain integrins can also bind to soluble ligands such as fibrinogen, or to counterreceptors on adjacent cells such as the intracellular adhesion molecules (ICAMs), leading to aggregation of cells. Ligands serve to cross-link or cluster integrins by binding to adjacent integrin receptors; both receptor clustering and ligand occupancy are necessary for the activation of integrin-mediated responses. In addition to mediating cell adhesion and cytoskeletal organization, integrins function as signaling receptors. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Integrin beta-1; Fibronectin receptor subunit beta; Glycoprotein IIa; GPIIA; VLA-4 subunit beta; CD29; ITGB1; FNRB; MDF2; MSK12

Swiss Prot

P05556

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 741-790 of Human Integrin β1.

Conjugation

Unconjugated

Related Pathways

Regulation of Actin Dynamics, Tumor Angiogenesis, Contribution of ECM and Cytoskeletal Factors to EMT

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 115 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

ITGB1 (Y783) polyclonal antibody detects endogenous levels of ITGB1 protein.

Applications Notes

Western blot (WB) analysis of ITGB1 pAb at 1:500 dilution Lane1:U-87MG whole cell lysate (40ug) Lane2:A375 whole cell lysate (40ug) Lane3:The Skin tissue lysate of Rat (40ug) Lane4:CT26 whole cell lysate (40ug)