ADORA3 (E318) polyclonal antibody

Product Specifications

Background

Adenosine is involved in a variety of processes, including the synthesis of urea, the anti-inflammatory response and the inhibition of protein synthesis The adenosine receptors, including adenosine A1-R, adenosine A2A-R, adenosine A2B-R and adenosine A3-R, are integral membrane proteins that are members of the G protein-coupled receptor family. The A1-R protein mediates ureagenesis in a partially calcium-dependent manner. Adenosine is known to mediate coronary vasodilation via the A2A-R receptor. Collagen synthesis and total protein synthesis are inhibited in certain cells by adenosine, acting via the A2B receptors. Activation of the A3-R receptor inhibits the induction of the cytokine TNFα and blocks the endotoxin CD14 receptor signal transduction pathway.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

ADORA3; ORF Names: UNQ1931/PRO4406; Adenosine receptor A3; Adenosine A3-R

Swiss Prot

P33765

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids C-terminus of Human ADORA3.

Conjugation

Unconjugated

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 38 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

ADORA3 (E318) polyclonal antibody detects endogenous levels of Adenosine receptor A3 protein.

Applications Notes

Western blot (WB) analysis of ADORA3 pAb at 1:500 dilution Lane1:C6 whole cell lysate (40ug) Lane2:SHSY5Y whole cell lysate (40ug) Lane3:SP2/0 whole cell lysate (40ug) Lane4:A549 whole cell lysate (40ug) Lane5:Jurkat whole cell lysate (40ug)