B-Myb (G573) polyclonal antibody

Product Specifications

Background

C-Myb is the cellular homolog of the leukemogenic avian retroviral protein v-Myc. c-Myb is expressed predominantly in immature and rapidly dividing hematopoietic cells, and cellular levels of c-Myb substantially decreases as cells reach terminal differentiation. B-Myb is expressed in a wide variety of proliferating cells, with levels accumulating during the G1 to S phase transition. A-Myb is expressed at specific times in reproductive tissues, some neural cells, and a subset of normal and neoplastic B lymphocytes.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Myb-related protein B; B Myb; Myb-like protein 2; MYBL2; BMYB

Swiss Prot

P10244

Reactivity

Human, Mouse

Immunogen

Synthetic peptide, corresponding to amino acids 543-590 of Human B-Myb.

Conjugation

Unconjugated

Applications

WB IF

Dilution

WB: 1:500~1:1000 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 78 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

B-Myb (G573/577) polyclonal antibody detects endogenous levels of B-Myb protein.

Applications Notes

Western blot (WB) analysis of MYBL2 pAb at 1:1000 dilution Lane1:Panc1 whole cell lysate (40ug) Lane2:MEF whole cell lysate (40ug) Lane3:SHSY5Y whole cell lysate (40ug) Lane4:Jurkat whole cell lysate (40ug) Lane5:K562 whole cell lysate (40ug)