NOLC1 polyclonal antibody

Product Specifications

Background

Nopp140, previously named p130, is a nucleolar phosphoprotein that has been shown to exist in multiple forms with different sizes. Nopp140 functions both as a chaperone for import and/or export from the nucleolus and as a transcription factor. Nopp140 was originally identified from rat liver as an NLS (nuclear localization signal) -binding protein, and has been further characterized as an RNAP (RNA Polymerase) -interacting protein. Nopp140 also associates with the general transcription factor TFIIB, and the protein kinase casein kinase II (CKII) . CKII heavily phosphorylates Nopp140 to mediate binding of Nopp140 to NLS. Nopp 140 colocalizes with another nucleolar protein, NAP57, in the nucleolus and coiled bodies, and is thought to be involved in activities carried out within the nucleolus.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Nucleolar and coiled-body phosphoprotein 1; 140 kDa nucleolar phosphoprotein; Nopp140; Hepatitis C virus NS5A-transactivated protein 13; HCV NS5A-transactivated protein 13; Nucleolar 130 kDa protein; Nucleolar phosphoprotein p130; KIAA0035; NS5ATP13

Swiss Prot

Q14978

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 654-699 of Human NOLC1.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 125 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

NOLC1 polyclonal antibody detects endogenous levels of NOLC1 protein.

Applications Notes

Western blot (WB) analysis of NOLC1 pAb at 1:500 dilution Lane1:CT-26 whole cell lysate (40ug) Lane2:C6 whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:Panc1 whole cell lysate (40ug) Lane5:SGC7901 whole cell lysate (40ug)